Specific interaction in biologic systems

Involved People: Iago Peters, Ulrich Ramach

Protein interaction in model membrane systems often differ from behavior seen by living cells. This, inb turn, makes it difficult to accurately mimic cell interaction. Here, with a combination of SFA and single-molecule AFM, we explore the differences in protein interaction behavior regarding surrounding glycocalyx proteins as well as the fluidity of the underlying tethered bilayer lipid membrane. This, in combination with the comparison of single-molecule interaction and ensemble behavior, allows for a deeper insight in where the limits of model systems are.